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ATCC acinetobacter species
(A) shows the alignment of sequence of diverse isolates of A. baumannii ( N =4), A. pittii ( N = 4), and A. nosocomialis (N=2) and demonstrates that the sequences are conserved across diverse isolates of three species except few locations. (B) shows the alignment of our consensus ITS 16S–23S rRNA sequence with the sequence of other <t>Acinetobacter</t> species ( A. calcoaceticus , A. lwoffii , A. radioresistens , A. haemolyticus , and A. junii ). The presence of the non-conserved region at locations from 140 to 210 (where our two to three primers are aligned) demonstrates that our LAMP primers were not specific for other Acinetobacter species.
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NCIMB Ltd ent. aerogenes ncimb
(A) shows the alignment of sequence of diverse isolates of A. baumannii ( N =4), A. pittii ( N = 4), and A. nosocomialis (N=2) and demonstrates that the sequences are conserved across diverse isolates of three species except few locations. (B) shows the alignment of our consensus ITS 16S–23S rRNA sequence with the sequence of other <t>Acinetobacter</t> species ( A. calcoaceticus , A. lwoffii , A. radioresistens , A. haemolyticus , and A. junii ). The presence of the non-conserved region at locations from 140 to 210 (where our two to three primers are aligned) demonstrates that our LAMP primers were not specific for other Acinetobacter species.
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Becton Dickinson fixable viability stain 700
(A) shows the alignment of sequence of diverse isolates of A. baumannii ( N =4), A. pittii ( N = 4), and A. nosocomialis (N=2) and demonstrates that the sequences are conserved across diverse isolates of three species except few locations. (B) shows the alignment of our consensus ITS 16S–23S rRNA sequence with the sequence of other <t>Acinetobacter</t> species ( A. calcoaceticus , A. lwoffii , A. radioresistens , A. haemolyticus , and A. junii ). The presence of the non-conserved region at locations from 140 to 210 (where our two to three primers are aligned) demonstrates that our LAMP primers were not specific for other Acinetobacter species.
Fixable Viability Stain 700, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) shows the alignment of sequence of diverse isolates of A. baumannii ( N =4), A. pittii ( N = 4), and A. nosocomialis (N=2) and demonstrates that the sequences are conserved across diverse isolates of three species except few locations. (B) shows the alignment of our consensus ITS 16S–23S rRNA sequence with the sequence of other Acinetobacter species ( A. calcoaceticus , A. lwoffii , A. radioresistens , A. haemolyticus , and A. junii ). The presence of the non-conserved region at locations from 140 to 210 (where our two to three primers are aligned) demonstrates that our LAMP primers were not specific for other Acinetobacter species.

Journal: Frontiers in Molecular Biosciences

Article Title: Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members of Acinetobacter calcoaceticus–baumannii Complex and Associated Carbapenem Resistance

doi: 10.3389/fmolb.2021.659256

Figure Lengend Snippet: (A) shows the alignment of sequence of diverse isolates of A. baumannii ( N =4), A. pittii ( N = 4), and A. nosocomialis (N=2) and demonstrates that the sequences are conserved across diverse isolates of three species except few locations. (B) shows the alignment of our consensus ITS 16S–23S rRNA sequence with the sequence of other Acinetobacter species ( A. calcoaceticus , A. lwoffii , A. radioresistens , A. haemolyticus , and A. junii ). The presence of the non-conserved region at locations from 140 to 210 (where our two to three primers are aligned) demonstrates that our LAMP primers were not specific for other Acinetobacter species.

Article Snippet: The specificity of LAMP assay was also determined using 21 non-ACB complex species [ A. lwoffii (6), A. variabilis (3), A. ursingii (2), A. radioresistens (2), A. junii (2), A. schindleri (2), A. indicus (2), A. haemolyticus (1), and A. bereziniae (1)] and 10 non- Acinetobacter species [seven reference strains ( Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC BAA 1705, Enterobacter cloacae ATCC 700323, Candida krusei ATCC 6258, and Pseudomonas aeruginosa ATCC 27858) and three clinical isolates ( Serratia marcescens , Providencia stuartii , and Stenotrophomonas maltophilia )].

Techniques: Sequencing

A universal consensus sequence of the ITS 16S–23S rRNA gene that was used to design the LAMP primers for detection of significant members of the Acinetobacter calcoaceticus–baumannii (ACB) complex. Red color bases signify the mixed bases used in place of different bases present in A. baumannii , A. nosocomialis , and A. pittii . The sequences sites for ITS Ab1 and Ab2 LAMP primers are designated by upper and lower horizontal arrows, respectively. Right and left arrows indicate sense and reverse complementary sequences that were used.

Journal: Frontiers in Molecular Biosciences

Article Title: Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members of Acinetobacter calcoaceticus–baumannii Complex and Associated Carbapenem Resistance

doi: 10.3389/fmolb.2021.659256

Figure Lengend Snippet: A universal consensus sequence of the ITS 16S–23S rRNA gene that was used to design the LAMP primers for detection of significant members of the Acinetobacter calcoaceticus–baumannii (ACB) complex. Red color bases signify the mixed bases used in place of different bases present in A. baumannii , A. nosocomialis , and A. pittii . The sequences sites for ITS Ab1 and Ab2 LAMP primers are designated by upper and lower horizontal arrows, respectively. Right and left arrows indicate sense and reverse complementary sequences that were used.

Article Snippet: The specificity of LAMP assay was also determined using 21 non-ACB complex species [ A. lwoffii (6), A. variabilis (3), A. ursingii (2), A. radioresistens (2), A. junii (2), A. schindleri (2), A. indicus (2), A. haemolyticus (1), and A. bereziniae (1)] and 10 non- Acinetobacter species [seven reference strains ( Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC BAA 1705, Enterobacter cloacae ATCC 700323, Candida krusei ATCC 6258, and Pseudomonas aeruginosa ATCC 27858) and three clinical isolates ( Serratia marcescens , Providencia stuartii , and Stenotrophomonas maltophilia )].

Techniques: Sequencing

Details of bacterial strains used in the study.

Journal: Frontiers in Molecular Biosciences

Article Title: Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members of Acinetobacter calcoaceticus–baumannii Complex and Associated Carbapenem Resistance

doi: 10.3389/fmolb.2021.659256

Figure Lengend Snippet: Details of bacterial strains used in the study.

Article Snippet: The specificity of LAMP assay was also determined using 21 non-ACB complex species [ A. lwoffii (6), A. variabilis (3), A. ursingii (2), A. radioresistens (2), A. junii (2), A. schindleri (2), A. indicus (2), A. haemolyticus (1), and A. bereziniae (1)] and 10 non- Acinetobacter species [seven reference strains ( Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC BAA 1705, Enterobacter cloacae ATCC 700323, Candida krusei ATCC 6258, and Pseudomonas aeruginosa ATCC 27858) and three clinical isolates ( Serratia marcescens , Providencia stuartii , and Stenotrophomonas maltophilia )].

Techniques: